4F0F
Crystal Structure of the Roco4 Kinase Domain bound to AppCp from D. discoideum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97780 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.720, 42.720, 339.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.100 - 1.800 |
| R-factor | 0.19769 |
| Rwork | 0.195 |
| R-free | 0.24512 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dtc |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.246 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0093) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.100 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 30175 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 100 mM Bis- Tris pH, 200 mM Na/K Tartrate; 11% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
