4EZD
Crystal Structure of the UT-B Urea Transporter from Bos Taurus Bound to Selenourea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2011-01-29 |
Detector | ADSC Q315R |
Wavelength(s) | 0.9788 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.002, 106.227, 105.816 |
Unit cell angles | 90.00, 98.18, 90.00 |
Refinement procedure
Resolution | 47.370 - 2.500 |
R-factor | 0.1988 |
Rwork | 0.197 |
R-free | 0.22850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.190 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
Rmerge | 0.168 | 0.102 | 0.921 |
Number of reflections | 56923 | ||
<I/σ(I)> | 6.8 | ||
Completeness [%] | 99.9 | 98.7 | 100 |
Redundancy | 7 | 7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 25% PEG 400, 50 mM sodium sulfate, 50 mM lithium sulfate, 0.2 mM DDAO, 100 mM Tris pH 8.0-8.5, 20 mM selenourea, VAPOR DIFFUSION, SITTING DROP, temperature 277K |