4EZ6
Bacillus DNA Polymerase I Large Fragment Complex 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Detector technology | CCD |
Collection date | 2009-10-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97121 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.740, 109.510, 149.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.870 - 1.640 |
R-factor | 0.1717 |
Rwork | 0.171 |
R-free | 0.19220 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2HVI WITH INSERTION SITE BASE PAIR METAL IONS AND PROTEIN RESIDUES 681-721 DELETED |
RMSD bond length | 0.010 |
RMSD bond angle | 1.355 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX (dev_1026) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.740 |
High resolution limit [Å] | 1.640 | 4.880 | 1.640 |
Rmerge | 0.055 | 0.025 | 0.457 |
Number of reflections | 166080 | ||
<I/σ(I)> | 16.63 | 46.53 | 3.63 |
Completeness [%] | 88.1 | 86.6 | 98.3 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 48%-53% Saturated Ammonium Sulfate, 2.5% MPD, 10mM MgSO4, 100mM MES (pH 5.8), VAPOR DIFFUSION, HANGING DROP, temperature 290K |