4EZ5
CDK6 (monomeric) in complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1 |
| Spacegroup name | I 4 |
| Unit cell lengths | 102.478, 102.478, 59.987 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.625 - 2.700 |
| R-factor | 0.2196 |
| Rwork | 0.216 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nup |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.948 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.9.3L) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.086 |
| Number of reflections | 8618 |
| <I/σ(I)> | 9.8 |
| Completeness [%] | 100.0 |
| Redundancy | 7.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M MES pH 6.0, 12% PEG-3350, 0.1M NH4NO3, EVAPORATION, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
