4EYM
MAPK13 complex with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2011-09-29 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0375 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.110, 69.564, 93.157 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.911 - 2.353 |
| R-factor | 0.2064 |
| Rwork | 0.203 |
| R-free | 0.26940 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.815 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.070 | 0.253 |
| Number of reflections | 15987 | |
| <I/σ(I)> | 23.8 | 5.5 |
| Completeness [%] | 93.6 | 99.9 |
| Redundancy | 5.4 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 50 mM ammonium tartrate, 18% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
