4EY2
Crystal structure of NDM-1 bound to hydrolyzed methicillin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-22 |
| Detector | RAYONIX MX300HE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.120, 79.360, 133.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.430 - 1.170 |
| R-factor | 0.131 |
| Rwork | 0.130 |
| R-free | 0.15900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.107 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.430 | 1.230 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Number of reflections | 137386 | |
| Completeness [%] | 96.8 | 93.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 298 | 0.2M MgCl2, 25% PEG3350, 0.1M bis-tris , pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
