4EXZ
Crystal Structure of the Q108K:K40L Mutant of Cellular Retinol Binding Protein Type II in Complex with All-trans-Retinal at 1.7 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.1272 |
Spacegroup name | P 1 |
Unit cell lengths | 29.814, 35.944, 63.975 |
Unit cell angles | 90.83, 92.88, 113.19 |
Refinement procedure
Resolution | 33.016 - 1.608 |
R-factor | 0.21524 |
Rwork | 0.212 |
R-free | 0.26511 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rcq |
RMSD bond length | 0.020 |
RMSD bond angle | 1.951 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 33.016 |
High resolution limit [Å] | 1.608 |
Number of reflections | 27686 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |