4EXZ

Crystal Structure of the Q108K:K40L Mutant of Cellular Retinol Binding Protein Type II in Complex with All-trans-Retinal at 1.7 Angstrom Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.1272
Spacegroup name	P 1
Unit cell lengths	29.814, 35.944, 63.975
Unit cell angles	90.83, 92.88, 113.19

Refinement procedure

Resolution	33.016 - 1.608
R-factor	0.21524
Rwork	0.212
R-free	0.26511
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rcq
RMSD bond length	0.020
RMSD bond angle	1.951
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.016
High resolution limit [Å]	1.608
Number of reflections	27686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1