4EWS
Crystal structure of cholesteryl ester transfer protein in complex with inhibitors
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.600, 69.323, 188.233 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.750 - 2.590 |
R-factor | 0.2155 |
Rwork | 0.213 |
R-free | 0.25910 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2obd |
RMSD bond length | 0.009 |
RMSD bond angle | 1.260 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.590 |
Number of reflections | 22976 |
Completeness [%] | 79.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 10mg/ml protein 1:1 mixing with 0.1M HEPES, pH=7.5, 0.2 M MgCl2 and 27-35% (W/v) PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 277K |