4EW1
High resolution structure of human glycinamide ribonucleotide transformylase in apo form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 78.028, 78.028, 230.841 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.570 - 1.522 |
| R-factor | 0.17385 |
| Rwork | 0.173 |
| R-free | 0.19304 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1meo |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.636 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.570 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Number of reflections | 64515 | |
| <I/σ(I)> | 41.4 | 3 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 9.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 298 | 0.1 M phosphate/citrate buffer, 1.5-2.0 M ammonium sulfate at p.H 4.2. 25% Glycerol added as cryoprotectant, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
