4EW0

mouse MBD4 glycosylase domain in complex with a G:5hmU (5-hydroxymethyluracil) mismatch

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	40.080, 110.230, 118.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.086 - 2.390
R-factor	0.2786
Rwork	0.278
R-free	0.29580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ngn
RMSD bond length	0.004
RMSD bond angle	0.741
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.090	2.480
High resolution limit [Å]	2.390	2.390
Rmerge	0.093	0.559
Number of reflections	10704
<I/σ(I)>	21.9	3.5
Completeness [%]	99.5	97.7
Redundancy	11.6	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	289	25% polyethylene glycol 3350, 200 mM NaCl, 100 mM BIS-TRIS-HCl, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K