4EV6
The complete structure of CorA magnesium transporter from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.110, 124.830, 111.880 |
| Unit cell angles | 90.00, 90.76, 90.00 |
Refinement procedure
| Resolution | 49.440 - 3.200 |
| R-factor | 0.2092 |
| Rwork | 0.205 |
| R-free | 0.28210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4egw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.506 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 46822 | |
| Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 30% PEG 8000, HEPES-NAOH, 60mM MgCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
