4EUM
Crystal structure of a sugar kinase (Target EFI-502132) from Oceanicola granulosus with bound AMP, crystal form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-19 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.540, 49.528, 83.267 |
Unit cell angles | 90.00, 101.50, 90.00 |
Refinement procedure
Resolution | 27.198 - 1.800 |
R-factor | 0.1786 |
Rwork | 0.176 |
R-free | 0.22070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lhx |
RMSD bond length | 0.007 |
RMSD bond angle | 1.109 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 81.595 | 77.943 | 1.900 |
High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
Rmerge | 0.039 | 0.645 | |
Total number of observations | 5443 | 26412 | |
Number of reflections | 56642 | ||
<I/σ(I)> | 8.8 | 16.7 | 1.2 |
Completeness [%] | 95.8 | 82.6 | 95.9 |
Redundancy | 3.4 | 3.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SITTING DROP, VAPOR DIFFUSION | 4 | 298 | protein (10 mM HEPES, pH 7.5, 150 mM sodium chloride, 10% glycerol, 1 mM DTT, 5 mM AMP-PNP), reservoir (0.2 M magnesium chloride, 0.1 M Bis-Tris, pH 6.5, 25% PEG3350), cryoprotectant (reservoir + 20% glycerol), SITTING DROP, VAPOR DIFFUSION, temperature 298K |