4ES0
X-ray structure of WDR5-SETd1b Win motif peptide binary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Detector technology | CCD |
Collection date | 2009-09-30 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.917 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 78.254, 98.272, 79.879 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.852 - 1.817 |
R-factor | 0.2158 |
Rwork | 0.214 |
R-free | 0.24210 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.154 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.870 |
High resolution limit [Å] | 1.810 | 3.900 | 1.810 |
Rmerge | 0.130 | 0.087 | 0.377 |
Number of reflections | 27686 | ||
<I/σ(I)> | 14.5 | ||
Completeness [%] | 98.3 | 97 | 89.8 |
Redundancy | 13.2 | 12.5 | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7.6 | 294 | PEG3350, Ammonium Sulfate, HEPES, pH 7.6, hanging drop, temperature 294K |