4EP7
Functional implications from the Cid1 poly(U) polymerase crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9811 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.490, 76.860, 81.860 |
| Unit cell angles | 90.00, 91.17, 90.00 |
Refinement procedure
| Resolution | 45.193 - 2.280 |
| R-factor | 0.1907 |
| Rwork | 0.188 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.112 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_353)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.193 | 4.000 | 2.300 |
| High resolution limit [Å] | 2.270 | 3.000 | 2.270 |
| Rmerge | 0.130 | ||
| Number of reflections | 29999 | ||
| <I/σ(I)> | 11.5 | 15.88 | 2.64 |
| Completeness [%] | 97.4 | 99.2 | 59.3 |
| Redundancy | 2.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 291 | 0.1M Imidatole/MES pH 6.1, 20% Glycerol, 10% PEG 4000, 42mM NaI, 42mM NaBr, 42mM NaF., VAPOR DIFFUSION, SITTING DROP, temperature 291K |
