4EP5
Thermus thermophilus RuvC structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 96 |
Detector technology | CCD |
Collection date | 2011-12-02 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 35.183, 60.019, 135.734 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.130 - 2.080 |
R-factor | 0.213 |
Rwork | 0.208 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.974 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1005)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.070 | 2.080 |
Rmerge | 0.060 | 0.698 |
Number of reflections | 8463 | |
<I/σ(I)> | 6.5 | 1.9 |
Completeness [%] | 93.3 | 0.7 |
Redundancy | 6.5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.2 | 293 | PEG400, Li2SO4, and sodium acetate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |