4EO4
Crystal structure of the yeast mitochondrial threonyl-tRNA synthetase (MST1) in complex with seryl sulfamoyl adenylate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 76 |
Detector technology | CCD |
Collection date | 2011-10-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 213.233, 107.498, 153.391 |
Unit cell angles | 90.00, 130.05, 90.00 |
Refinement procedure
Resolution | 49.722 - 2.870 |
R-factor | 0.1777 |
Rwork | 0.175 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.750 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.930 |
High resolution limit [Å] | 2.870 | 2.870 |
Number of reflections | 60275 | |
<I/σ(I)> | 15 | 2.2 |
Completeness [%] | 99.8 | 97.7 |
Redundancy | 5.6 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 285 | 0.1M di-sodium phosphate / mono-potassium phosphate, 0.2M NaCl, 10 % (w/v) PEG 8,000, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 285K |