4ENO
Crystal structure of oxidized human nm23-H1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1 |
Spacegroup name | P 21 3 |
Unit cell lengths | 106.768, 106.768, 106.768 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.748 - 2.800 |
R-factor | 0.1895 |
Rwork | 0.182 |
R-free | 0.25590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.315 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.755 | |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 10255 | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 296 | 2.0M sodium potassium phosphate pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K |