4ENK

Crystal structure of S. pombe Atl1 in complex with damaged DNA containing O6-propylguanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-19
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1158
Spacegroup name	P 61 2 2
Unit cell lengths	59.501, 59.501, 236.917
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.879 - 3.045
R-factor	0.2544
Rwork	0.250
R-free	0.29520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gva
RMSD bond length	0.010
RMSD bond angle	1.651
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.160
High resolution limit [Å]	3.045	3.045
Number of reflections	5247
Completeness [%]	97.6	82.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	288	25% mPEG 2000, 5% sodium formate, 0.2M imidazole-malate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 288K