4EMO
Crystal structure of the PH domain of SHARPIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2009-05-29 |
Detector | ADSC |
Wavelength(s) | 0.97990 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 61.550, 61.550, 222.813 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.2122 |
Rwork | 0.209 |
R-free | 0.26910 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 2.135 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | RESOLVE (2.13) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.090 |
High resolution limit [Å] | 2.000 | 4.000 | 2.000 |
Rmerge | 0.071 | 0.042 | 0.483 |
Number of reflections | 29888 | ||
<I/σ(I)> | 10.7 | ||
Completeness [%] | 99.1 | 96.6 | 98.3 |
Redundancy | 3.9 | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 4M sodium formate, pH 7.4, vapor diffusion, sitting drop, temperature 291K |