4EME
X-ray crystal structure and specificity of the Plasmodium falciparum malaria aminopeptidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.954 |
Spacegroup name | P 21 3 |
Unit cell lengths | 200.384, 200.384, 200.384 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.580 - 2.600 |
R-factor | 0.1613 |
Rwork | 0.159 |
R-free | 0.19590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.140 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.400 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.248 | 1.439 |
Number of reflections | 82063 | |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 9.6 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 67% MPD, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 100K |