4EMA

Human peroxisome proliferator-activated receptor gamma in complex with rosiglitazone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.46
Spacegroup name	C 1 2 1
Unit cell lengths	90.511, 61.958, 117.601
Unit cell angles	90.00, 100.70, 90.00

Refinement procedure

Resolution	28.890 - 2.545
R-factor	0.1821
Rwork	0.180
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.583
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.890	2.640
High resolution limit [Å]	2.540	2.540
Number of reflections	20835
Completeness [%]	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1M tris-HCl, 0.9M sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K