4ELV
Snapshot of the large fragment of DNA polymerase I from Thermus Aquaticus processing modified pyrimidines
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-08-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 109.213, 109.213, 91.047 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.291 - 1.900 |
R-factor | 0.1785 |
Rwork | 0.176 |
R-free | 0.21570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ojs |
RMSD bond length | 0.007 |
RMSD bond angle | 1.172 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (dev_1009) |
Refinement software | PHENIX ((phenix.refine: dev_1009)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.118 | 1.182 |
Number of reflections | 50305 | |
<I/σ(I)> | 1.2 | |
Completeness [%] | 99.3 | 95.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 291 | 50 mM Tris HCl pH=8.4, 0.2 M NH4Cl, 10 mM CaCl2, 34% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |