4ELL
Structure of the inactive retinoblastoma protein pocket domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0971 |
Spacegroup name | H 3 |
Unit cell lengths | 249.659, 249.659, 35.110 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.035 - 1.980 |
R-factor | 0.1943 |
Rwork | 0.193 |
R-free | 0.23770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.996 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.180 | 2.080 |
High resolution limit [Å] | 1.980 | 1.980 |
Number of reflections | 57109 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277.15 | 100mM sodium citrate, 1mM LiCl, 18% PEG 8K., pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |