4EL9
Structure of N-terminal kinase domain of RSK2 with afzelin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-30 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9712 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 99.119, 40.860, 83.867 |
Unit cell angles | 90.00, 114.64, 90.00 |
Refinement procedure
Resolution | 16.710 - 1.550 |
R-factor | 0.2264 |
Rwork | 0.200 |
R-free | 0.22640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ubd |
RMSD bond length | 0.006 |
RMSD bond angle | 0.939 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.000 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 43153 | |
<I/σ(I)> | 23.2 | 2 |
Completeness [%] | 96.8 | 76.9 |
Redundancy | 4.1 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 30% Jeffamine ED2003, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |