4EKY
Crystal structure of GPb in complex with DK15
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2011-10-07 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1.5419 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.674, 128.674, 116.564 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.570 - 2.450 |
R-factor | 0.16435 |
Rwork | 0.161 |
R-free | 0.23038 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3sym |
RMSD bond length | 0.018 |
RMSD bond angle | 1.771 |
Data reduction software | CrysalisPro |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.700 | 2.580 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 33602 | |
<I/σ(I)> | 13.8 | 2.3 |
Completeness [%] | 82.4 | |
Redundancy | 5.5 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 10mM BES buffer, pH 6.7, SMALL TUBES, temperature 289K |