4EKK
Akt1 with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-12-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.637, 55.853, 91.402 |
| Unit cell angles | 90.00, 103.43, 90.00 |
Refinement procedure
| Resolution | 29.690 - 2.800 |
| R-factor | 0.2202 |
| Rwork | 0.217 |
| R-free | 0.27998 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.096 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.190 | 0.390 |
| Number of reflections | 21128 | |
| <I/σ(I)> | 5.7 | 2.4 |
| Completeness [%] | 99.3 | 96.6 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 20% PEG4K, 100mM Tris-pH7.5, Under Oil, temperature 293K |
