4EIK
Crystal Structure of the Human Fyn SH3 domain in complex with the synthetic peptide VSL12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-24 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97 |
Spacegroup name | P 63 |
Unit cell lengths | 81.843, 81.843, 35.904 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.658 - 1.600 |
R-factor | 0.1752 |
Rwork | 0.174 |
R-free | 0.19540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ua7 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.928 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.658 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.031 | 0.230 |
Number of reflections | 17446 | |
<I/σ(I)> | 71.1 | 10 |
Completeness [%] | 94.4 | 72.5 |
Redundancy | 11 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 5.5 M sodium formate, 0.1 M MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |