4EHC
Crystal structure of the C-terminal domain of Rv0977 of Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.110, 66.160, 69.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.728 - 1.980 |
| R-factor | 0.1674 |
| Rwork | 0.165 |
| R-free | 0.21262 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.623 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.6.0059) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.728 | 2.080 |
| High resolution limit [Å] | 1.975 | 1.980 |
| Rmerge | 0.132 | 0.415 |
| Number of reflections | 20442 | |
| <I/σ(I)> | 16.3 | 6.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.6 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 293 | 0.1M MES sodium salt pH 6.5, 12% PEG 20000, Microbatch, temperature 293K |
