4EG2
2.2 Angstrom Crystal Structure of Cytidine deaminase from Vibrio cholerae in Complex with Zinc and Uridine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97959 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.810, 163.730, 111.950 |
| Unit cell angles | 90.00, 97.70, 90.00 |
Refinement procedure
| Resolution | 40.210 - 2.200 |
| R-factor | 0.16178 |
| Rwork | 0.159 |
| R-free | 0.21039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ctu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.319 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.087 | 0.615 |
| Number of reflections | 116604 | |
| <I/σ(I)> | 6.7 | 2.07 |
| Completeness [%] | 97.3 | 92.9 |
| Redundancy | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | Protein solution: 0.3M Sodium chloride, 10mM HEPES. Screen solution: 0.2M MgCl2, 0.1M Hepes, 10mM Cytidine, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
