4EEE
Crystal Structure of YLDV 14L IL-18 Binding Protein in Complex with Human IL-18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97911 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.380, 41.771, 104.431 |
Unit cell angles | 90.00, 98.27, 90.00 |
Refinement procedure
Resolution | 37.270 - 2.710 |
R-factor | 0.224 |
Rwork | 0.219 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f62 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.904 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.507 | |
Number of reflections | 18239 | |
<I/σ(I)> | 1.8 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 18% PEG 3350, 0.1M BIS-TRIS PROPANE/CITRIC ACID PH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |