4EDK

The structure of the S. aureus DnaG RNA Polymerase Domain bound to GTP and Manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115
Spacegroup name	P 61
Unit cell lengths	151.742, 151.742, 38.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.935 - 2.000
R-factor	0.1627
Rwork	0.161
R-free	0.19990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.932
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	PHENIX (AutoMR)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.935	43.804	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge		0.052	0.495
Total number of observations		23696	54585
Number of reflections	35005
<I/σ(I)>	21.8	12.4	1.5
Completeness [%]	100.0	99.8	100
Redundancy	14.5	19.9	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	0.15M sodium thiocyanate, 0.1M Bis-Tris, 13% PEG3350, 2% Benzamidine, 2.5 mM GTP, 5 mM MnCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K