4ECC
Chimeric GST Containing Inserts of Kininogen Peptides
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97857 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 92.520, 92.520, 58.087 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.22231 |
Rwork | 0.220 |
R-free | 0.26892 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m99 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.363 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
Number of reflections | 12806 | ||
Completeness [%] | 100.0 | 91 | 97.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | (1)0.2 M Ammonium Sulfate, 0.1 M Hepes, 25% PEG 3350 (2)0.2 M Lithium Sulfate, 25% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |