4EC2
Crystal structure of trimeric frataxin from the yeast Saccharomyces cerevisiae, complexed with ferrous
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.038 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 121.570, 121.570, 121.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.815 - 3.002 |
| R-factor | 0.2597 |
| Rwork | 0.254 |
| R-free | 0.31130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fql |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.271 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.064 | 0.632 |
| Number of reflections | 11254 | |
| <I/σ(I)> | 11.62 | 1.76 |
| Completeness [%] | 96.9 | 93.3 |
| Redundancy | 3.32 | 3.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | 1.7 M (NH4)2SO4, 0.2 M Li2SO4, 4 % Gamma-butyrolactone, 0.1 M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
