4EA5
Structure of the glycoslyase domain of MBD4 bound to a 5hmU containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | .9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.490, 99.760, 53.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.130 - 2.140 |
| R-factor | 0.1948 |
| Rwork | 0.191 |
| R-free | 0.23530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e9e |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.130 | 2.270 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 11828 | |
| Completeness [%] | 99.2 | 95.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 20% Jeffamine M2070, 20% dimethylsulfoxite, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
