4E9G
structure of the glycosylase domain of MBD4 bound to thymine containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | .9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.080, 61.470, 96.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.970 - 2.350 |
| R-factor | 0.2106 |
| Rwork | 0.205 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e9e |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.490 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 10209 | |
| Completeness [%] | 98.0 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 15% PEG MME 2000, 100 mM MES, 0.2 M NaAcetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
