4E93
Crystal structure of human Feline Sarcoma Viral Oncogene Homologue (v-FES)in complex with TAE684
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-29 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.970, 35.330, 100.720 |
| Unit cell angles | 90.00, 96.58, 90.00 |
Refinement procedure
| Resolution | 27.969 - 1.840 |
| R-factor | 0.1885 |
| Rwork | 0.186 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bkb |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.726 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.930 | 27.969 | 1.940 |
| High resolution limit [Å] | 1.840 | 5.820 | 1.840 |
| Rmerge | 0.084 | 0.031 | 0.721 |
| Total number of observations | 5261 | 22108 | |
| Number of reflections | 33290 | ||
| <I/σ(I)> | 12.5 | 14.7 | 1.1 |
| Completeness [%] | 98.0 | 99.1 | 95.9 |
| Redundancy | 4.8 | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1M SPG, 30.0% PEG 1K, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
