4E7H

PFV intasome prior to 3'-processing, Apo form (UI-Apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0389
Spacegroup name	P 41 21 2
Unit cell lengths	159.470, 159.470, 123.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.916 - 2.570
R-factor	0.188
Rwork	0.186
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oy9
RMSD bond length	0.009
RMSD bond angle	1.364
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.710
High resolution limit [Å]	2.570	2.570
Rmerge	0.085	0.849
Number of reflections	50886
<I/σ(I)>	12.9	2
Completeness [%]	99.5	100
Redundancy	5.1	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	1.35 M AMMONIUM SULFATE, 25% (V/V), GLYCEROL, 4.8% (V/V) 1,6-HEXANEDIOL, 50 MM MES-NAOH, 2MM EDTA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K