4E7H
PFV intasome prior to 3'-processing, Apo form (UI-Apo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0389 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 159.470, 159.470, 123.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.916 - 2.570 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oy9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.364 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.710 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.085 | 0.849 |
| Number of reflections | 50886 | |
| <I/σ(I)> | 12.9 | 2 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.35 M AMMONIUM SULFATE, 25% (V/V), GLYCEROL, 4.8% (V/V) 1,6-HEXANEDIOL, 50 MM MES-NAOH, 2MM EDTA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
