4E75
Structure of LpxD from Acinetobacter baumannii at 2.85A resolution (P21 form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-15 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 106.679, 209.614, 107.633 |
Unit cell angles | 90.00, 119.23, 90.00 |
Refinement procedure
Resolution | 48.150 - 2.850 |
R-factor | 0.23668 |
Rwork | 0.234 |
R-free | 0.27988 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pm0 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.444 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.940 |
High resolution limit [Å] | 2.840 | 2.840 |
Rmerge | 0.113 | 0.585 |
Number of reflections | 94647 | |
<I/σ(I)> | 8.7 | 1.8 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 2ul of 15mg/ml protein, 2ul of 24% PEG3350, 0.2M Ammonium formate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |