4E69
Crystal structure of a sugar kinase (target EFI-502132) from Oceanicola granulosus, unliganded structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.249, 76.306, 76.245 |
| Unit cell angles | 90.00, 105.80, 90.00 |
Refinement procedure
| Resolution | 28.024 - 1.600 |
| R-factor | 0.1693 |
| Rwork | 0.168 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lhx |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.004 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.073 | 0.837 |
| Number of reflections | 84535 | |
| <I/σ(I)> | 9 | 1.6 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | Protein (10 mM HEPES pH 7.5, 150 mM NaCl, 10% glycerol, 1 mM DTT; Reservoir (1 M LiCl, 0.1 M NaCitrate pH 4, 20% Peg 6000); Cryoprotection (Reservoir, + 20% Ethyelene Glycol), VAPOR DIFFUSION, SITTING DROP, temperature 298K |
