4E5Y
Structure of human FX protein, the key enzyme in the biosynthesis of GDP-L-fucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97883 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.772, 136.440, 139.849 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.267 - 2.370 |
R-factor | 0.2341 |
Rwork | 0.232 |
R-free | 0.29150 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.366 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.410 |
High resolution limit [Å] | 2.370 | 2.370 |
Number of reflections | 67223 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 100mM Tris pH 8.0, 30% PEG 2000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |