4E5Y

Structure of human FX protein, the key enzyme in the biosynthesis of GDP-L-fucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97883
Spacegroup name	P 21 21 21
Unit cell lengths	85.772, 136.440, 139.849
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.267 - 2.370
R-factor	0.2341
Rwork	0.232
R-free	0.29150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.366
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.410
High resolution limit [Å]	2.370	2.370
Number of reflections	67223

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	100mM Tris pH 8.0, 30% PEG 2000, VAPOR DIFFUSION, SITTING DROP, temperature 277K