4E4H
Crystal structure of Histone Demethylase NO66
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 32 |
| Unit cell lengths | 89.352, 89.352, 304.863 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.280 |
| R-factor | 0.20418 |
| Rwork | 0.202 |
| R-free | 0.25186 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | selenomethionine labeled NO66 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.551 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.280 | 2.290 |
| Number of reflections | 123682 | |
| Completeness [%] | 98.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 200mM (NH4)2SO4, 100mM Tris-HCl, 10% PEG 8000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
