4E1J
Crystal structure of glycerol kinase in complex with glycerol from Sinorhizobium meliloti 1021
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.220, 101.975, 107.548 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 47.570 - 2.330 |
| R-factor | 0.23311 |
| Rwork | 0.230 |
| R-free | 0.28993 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.671 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.060 | 0.580 |
| Number of reflections | 86871 | |
| <I/σ(I)> | 7.9 | 2 |
| Completeness [%] | 99.3 | 93.4 |
| Redundancy | 7.2 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2M Na-malonate, pH 7.0, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
