4E1I
Fragment of human prion protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-13 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.338, 41.217, 46.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.300 - 2.030 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.25300 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.225 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.873 | 41.217 | 2.140 |
| High resolution limit [Å] | 2.028 | 6.410 | 2.028 |
| Rmerge | 0.038 | 0.196 | |
| Total number of observations | 914 | 2312 | |
| Number of reflections | 4685 | ||
| <I/σ(I)> | 20.1 | 16.1 | 3.9 |
| Completeness [%] | 98.6 | 98.4 | 90.7 |
| Redundancy | 5.7 | 5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 100mM Bis Tris pH 5.5, 25% PEG 3350, vapor diffusion, hanging drop, temperature 298K |
