4E17

Alpha-E-catenin is an autoinhibited molecule that co-activates vinculin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	193
Detector technology	CCD
Collection date	2011-11-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	50.747, 55.969, 128.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.133 - 2.304
R-factor	0.218
Rwork	0.216
R-free	0.24050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t01
RMSD bond length	0.003
RMSD bond angle	0.739
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.078	0.036	0.562
Number of reflections	16376
<I/σ(I)>	11.2
Completeness [%]	97.7	91.7	95.8
Redundancy	3.5	3.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	283	20% PEG8000, 100 mM MES, pH 6.8, 3 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 283K