4E0X

Crystal structure of the kainate receptor GluK1 ligand-binding domain in complex with kainate in the absence of glycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	110
Detector technology	CCD
Collection date	2011-04-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	68.256, 68.256, 235.095
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.513 - 2.000
R-factor	0.1769
Rwork	0.175
R-free	0.21860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c32
RMSD bond length	0.008
RMSD bond angle	1.004
Data reduction software	XDS
Data scaling software	SCALA (3.3.9)
Phasing software	PHASER
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.513	29.513	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge		0.025	0.411
Total number of observations		7832	35466
Number of reflections	38773
<I/σ(I)>	9.2	20.9	1.8
Completeness [%]	100.0	98.9	100
Redundancy	6.4	5.6	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	280	20% PEG4000, 0.35M lithium sulfate, 0.1M sodium cacodylate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 280K