4DXS
Human SUN2-KASH2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 79.320, 79.320, 260.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.060 - 2.710 |
| R-factor | 0.188 |
| Rwork | 0.182 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dxt |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.732 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_975)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.300 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.055 | 0.695 |
| Number of reflections | 8912 | |
| <I/σ(I)> | 21 | 2.7 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 5.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | 0.1 M HEPES, pH 7.4, 7% PEG4000, 10% 1,6-hexanediol, 0.25% DM, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
