4DXD
Staphylococcal Aureus FtsZ in complex with 723
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-08-05 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 67.715, 54.650, 84.496 |
Unit cell angles | 90.00, 106.75, 90.00 |
Refinement procedure
Resolution | 40.460 - 2.010 |
R-factor | 0.1892 |
Rwork | 0.188 |
R-free | 0.22160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.340 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.9.7) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.460 |
High resolution limit [Å] | 2.010 |
Number of reflections | 19629 |
Completeness [%] | 97.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 293 | 0.1M ammonium sulfate, 0.1M TRIS pH8.5 and 25% PEG3350, VAPOR DIFFUSION, temperature 293K |