4DWR
RNA ligase RtcB/Mn2+ complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-07-16 |
| Detector | APEX II CCD |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.081, 84.899, 124.200 |
| Unit cell angles | 90.00, 115.65, 90.00 |
Refinement procedure
| Resolution | 46.740 - 1.480 |
| R-factor | 0.14365 |
| Rwork | 0.142 |
| R-free | 0.17794 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.316 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.540 |
| Number of reflections | 294058 | |
| Completeness [%] | 95.0 | 62.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 1.5 M (NH4)2SO4, 75 mM NaCl, and 0.1 M HEPES-NaOH pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
