4DWQ
RNA ligase RtcB-GMP/Mn(2+) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-10-18 |
| Detector | APEX II CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.296, 137.756, 148.408 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 101.290 - 2.250 |
| R-factor | 0.15182 |
| Rwork | 0.150 |
| R-free | 0.19349 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.625 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 69979 | |
| Completeness [%] | 95.0 | 94.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2 M Na-Malonate pH 7.0, 20% glycerol, 10 mM MnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
