4DWQ
RNA ligase RtcB-GMP/Mn(2+) complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2011-10-18 |
Detector | APEX II CCD |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.296, 137.756, 148.408 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 101.290 - 2.250 |
R-factor | 0.15182 |
Rwork | 0.150 |
R-free | 0.19349 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.625 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 69979 | |
Completeness [%] | 95.0 | 94.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2 M Na-Malonate pH 7.0, 20% glycerol, 10 mM MnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |