4DW3
Crystal structure of the glycoprotein Erns from the pestivirus BVDV-1 in complex with 5'-CMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2008-11-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.930, 106.460, 64.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.330 - 2.350 |
| R-factor | 0.1879 |
| Rwork | 0.186 |
| R-free | 0.22110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dvk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.930 | 2.360 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 19939 | |
| Completeness [%] | 96.0 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 298 | 33% PEG2000 MME, 100mM Na-Acetate, 140mM (NH4)2SO4, 50mM KH2PO4, pH 4.6, vapor diffusion, temperature 298K |
